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Expression of Hsp70 and survival of human peripheral blood monocytes in response to in vitro exposure to Mycobacterium tuberculosis

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dc.contributor.advisor Dr. L. Bornman en_US
dc.contributor.author Mafoko, Baatseba
dc.date.accessioned 2012-08-27T12:24:30Z
dc.date.available 2012-08-27T12:24:30Z
dc.date.issued 2012-08-27
dc.date.submitted 2000
dc.identifier.uri http://hdl.handle.net/10210/6646
dc.description M.Sc. en_US
dc.description.abstract The induction of heat shock proteins (HSPs) in human monocytes during a pathogen challenge is a sophisticated selective response and plays an important role in cytoprotection from inflammation-related stress, including oxidative injury. We investigated the accumulation of the inducible isoform of the 70 kDa HSP, Hsp70, in peripheral blood monocytes from 12 healthy donors in response to Mycobacterium tuberculosis (M.tb) using flow cytometry, biometabolic labeling or Western blot analysis. Cells from each donor, prepared on two different occasions, were exposed to virulent (H37Rv) and attenuated (H37Ra) strains of M.tb at two bacterium : monocyte ratios (1:1 and 10:1) for 3 h and allowed to recover for an additional 2 h or 24 h. In spite of a prominent inter-individual variation, H37Ra (1:1, 2 h) significantly induced the mean Hsp70 accumulation (p<0.05) compared to normal cells, while H37Rv (10:1, 24 h) significantly suppressed the mean Hsp70 levels (p<0.001) in monocyte compared to normal monocytes or monocytes exposed to H37Ra. Survival of H37Rv-infected monocytes showed a significant correlation with Hsp70 levels. These results suggest a protective role of Hsp70 in host defense against mycobacterial infection. Cell death due to insufficient endogenous levels of Hsp70 implies a novel pathogenic strategy for virulence of M. tuberculosis. en_US
dc.language.iso en en_US
dc.subject Heat shock proteins en_US
dc.subject Monocytes en_US
dc.subject Tuberculosis - South Africa en_US
dc.subject Tuberculosis - South Africa - Prevention en_US
dc.title Expression of Hsp70 and survival of human peripheral blood monocytes in response to in vitro exposure to Mycobacterium tuberculosis en_US
dc.type Thesis en_US


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