Laser irradiation of adipose derived stem cells and their differentiation into smooth muscle cells

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dc.contributor.advisor Prof. H. Abrahamse; Dr. N.N. Houreld en_US
dc.contributor.author De Villiers, Jennifer Anne
dc.date.accessioned 2012-05-30T05:57:20Z
dc.date.available 2012-05-30T05:57:20Z
dc.date.issued 2012-05-30
dc.date.submitted 2010
dc.identifier.uri http://hdl.handle.net/10210/4742
dc.description M. Tech. en_US
dc.description.abstract Stem cells are regarded as undifferentiated cells that are capable of selfrenewal, proliferation, production of a great number of differentiated progeny, and regeneration of tissues (Blau et al., 2001). The therapeutic potential of multilineage stem cells for tissue engineering (TE) applications is vast. Two general types of stem cells are potentially useful for this application: embryonic stem cells (ESCs) and adult (autologous) stem cells (Zuk et al., 2001). Traditionally, ESCs are isolated from the inner cell mass (ICM) of blastocysts, however harvesting of these cells results in the death of the embryo, which has led to ethical, religious and political issues (Moore, 2007). In contrast, adult stem cells, by virtue of their nature, are immunocompatible and have no ethical issues associated with their use (Zuk et al., 2001). Subcutaneous adipose tissue is an active and highly complex tissue composed of several different cell types, and is derived from the mesodermal germ layer and contains a supportive stromal vascular fraction (SVF) that can be easily isolated. This SVF contains a heterogeneous mixture of cells including preadipocytes (Raposio et al., 2007; Schäffler and Büchler, 2007; Jurgens et al., 2008). The preadipoctyes are considered as the multipotent stem cells termed adipose derived stem cells (ADSCs) that have similar properties to bone marrow mesenchymal stem cells (BM-MSCs) (Fraser et al., 2006). ADSCs are idyllic for cellular therapy applications due to various factors: they can be harvested, multiplied and handled easily, efficiently and noninvasively, they have a pluripotential and proliferative capacity comparable to BM-MSCs, and morbidity to donors is considerably less, requiring only local anaesthesia and a short wound healing time. Human ADSCs (hADSCs) can be expanded in an undifferentiated state and have multipotential differentiation capacity along the classical mesenchymal lineages of adipogenesis, osteogenesis, chondrogenesis and myogenesis (de Villiers et al., 2009). en_US
dc.language.iso en en_US
dc.subject Stem cells transplantation en_US
dc.subject Phototherapy en_US
dc.subject Lasers en_US
dc.title Laser irradiation of adipose derived stem cells and their differentiation into smooth muscle cells en_US
dc.type Thesis en_US

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