Quality assessment of cryopreserved spermatozoa of the blesbok (Damaliscus pygargus phillipsi), blue wildebeest (Connochaetes taurinus) and African buffalo (Syncerus caffer)

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dc.contributor.advisor Dr. G.M. Wagenaar; Prof. P. Bartels en_US
dc.contributor.author Mynhardt, Neil Philip
dc.date.accessioned 2012-08-22T06:17:30Z
dc.date.available 2012-08-22T06:17:30Z
dc.date.issued 2012-08-22
dc.date.submitted 2012-05-23
dc.identifier.uri http://hdl.handle.net/10210/6360
dc.description M.Sc. en_US
dc.description.abstract Climate change, loss of habitat and over-exploitation of natural resources as well as the introduction of invasive alien species through human activities are resulting in an ever increasing risk of extinction of many plant and animal species. There are two major approaches to conserving threatened and endangered species. Firstly the large scale preservation of natural habitat and ecological processes, thereby protecting the species inhabiting the habitat. The second approach involves the ex-situ breeding of rare and endangered species. It is estimated that in the next 200 years approximately 800 mammalian species will require the assistance of breeding programs to ensure long term genetic viability. Biological Resource Banks (BRB) can potentially contribute to this challenge by providing a source of genes that can be used to counter the effects of external selection pressures, genetic drift and inbreeding depression in small or fragmented populations. These banks commonly contain biological materials such as cryopreserved sperm, embryos and cell cultures mainly as genetic and research resources. . Biological resource banks can potentially use these cryopreserved gametes together with assisted reproductive technologies (ART), such as artificial insemination (AI), in vitro fertilisation (IVF), embryo transfer (ET), intracytoplasmic sperm injection (ICSI) and nuclear transfer (NT) to maintain genetic heterogeneity in ex-situ and wild populations. Ascertaining the appropriate protocols for developing the ARTs necessary for non-domestic species is one of the major challenges faced by reproductive physiologists. Typically, there is very little available information about the processing of semen, the effects of diluents, concentration and type of cryoprotectants and freeze-thaw methods for sperm samples of non-domestic species. Procedures proven to be highly effective in humans and laboratory or domestic species, are frequently adopted and modified for use in related wildlife species. It is thus necessary to gain knowledge of the reproductive physiology of wildlife species in order to define effective protocols for the cryopreservation of biomaterials which assists in the conservation of South Africa‘s diverse wildlife species. Sperm quality assessment is a useful tool for assessing the reproductive health of free-ranging populations as well as for selecting individuals for future assisted reproduction programs. en_US
dc.language.iso en en_US
dc.subject Reproductive technology en_US
dc.subject Cryopreservation of organs, tissues, etc. en_US
dc.subject Game protection en_US
dc.subject Endangered species conservation en_US
dc.subject Blesbok - Germplasm resources - Cryopreservation
dc.subject Brindled gnu - Germplasm resources - Cryopreservation
dc.subject African buffalo- Germplasm resources - Cryopreservation
dc.subject Blesbok - Artificial insemination
dc.subject Brindled gnu - Artificial insemination
dc.subject African buffalo - Artificial insemination
dc.subject Extinction (Biology)
dc.title Quality assessment of cryopreserved spermatozoa of the blesbok (Damaliscus pygargus phillipsi), blue wildebeest (Connochaetes taurinus) and African buffalo (Syncerus caffer) en_US
dc.type Thesis en_US

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