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The in vitro propagation of Sparaxis sp. and other related genera

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dc.contributor.advisor Prof. K.T. van Warmelo en_US
dc.contributor.author Lundie, Vanessa
dc.date.accessioned 2012-09-06T05:34:42Z
dc.date.available 2012-09-06T05:34:42Z
dc.date.issued 2012-09-06
dc.date.submitted 1996
dc.identifier.uri http://hdl.handle.net/10210/7060
dc.description M.Sc. en_US
dc.description.abstract The family lridaceae includes genera such as Sparaxis, Freesia and Babiana which are popular flowering plants. The purpose of this study was the propagation in tissue culture of Sparaxis plants. After the applicable techniques have been formulated, the aim was to test the efficiency of the techniques on the other genera. The techniques can then be extended to endangered genera. Apical meristems as well as axillary meristems were dissected aseptically and grown on the medium of Heinz and Mee (1969). It is difficult to acquire aseptic explant material of Sparaxis because of the net-like fibres surrounding the corm. Growth of aseptic meristem explants occurred within a week under 16h light and 25 ± 1 °C. Proliferation of meristems occurred and gave rise to more than one plant from the same explant. The study was extended to seeds as an explant source, as well as the influences of various factors such as pH, carbon source and gelling agents, on the growth and proliferation of the plants. Rooting of the plants was investigated and hardening done for transfer of the plants to the greenhouse. en_US
dc.language.iso en en_US
dc.subject Iridaceae en_US
dc.subject Plant micropropagation en_US
dc.subject Fertilization of plants en_US
dc.subject Plant cell culture en_US
dc.title The in vitro propagation of Sparaxis sp. and other related genera en_US
dc.type Thesis en_US

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