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The in vitro propagation of species of Lotononis

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dc.contributor.advisor Prof. K.T. van Warmelo en_US
dc.contributor.author O'Brien, Oloff Marie
dc.date.accessioned 2012-09-11T08:03:20Z
dc.date.available 2012-09-11T08:03:20Z
dc.date.issued 2012-09-11
dc.date.submitted 1996
dc.identifier.uri http://hdl.handle.net/10210/7407
dc.description M.Sc. en_US
dc.description.abstract Lotononis bainesii Baker and Lotononis listii Polhill both belong to the subsection Listieae (Fabaceae). Lotononis bainesii is utilised as a forage legume in certain parts of the world, but other species in the genus may also have potential use as forage. The influence of medium composition and pH on multiplication and rooting, as well as hardening and Rhizobium nodulation, was investigated for both species. Shoots were induced in L. bainesii on modified MS media (De Fossard, 1976), supplemented with 0.025 to lOmmoll i NAA and IAA, 0.025 to 0.5/Arno& BAP and Kinetin, 2% fructose, 0.6% agar, with the pH adjusted to 5.8. L. listii was regenerated on the same basic media, but containing 2% sucrose, 0.4% Gelrite and pH adjusted to 5.4. Rooting of L. listii were accomplished on modified MS medium supplemented with 0.5Amol.1-1 IBA and IAA, and 0.025/anol.14 BAP and Kinetin. Rooting and nodule formation under natural conditions were successful for L. listii, but less successful for L. bainesii. Possible differences between plants cultured in vitro and under natural conditions, were also examined using scanning electron- and light microscopy. en_US
dc.language.iso en en_US
dc.subject Fertilization of plants en_US
dc.subject Plant micropropagation en_US
dc.subject Legumes en_US
dc.subject Lotononis bainesii en_US
dc.subject Lotononis listii en_US
dc.subject Tissue culture en_US
dc.title The in vitro propagation of species of Lotononis en_US
dc.type Thesis en_US

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