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A deep gold mine metagenome as a source of novel esterases.

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dc.contributor.author Abbai, Nathlee S.
dc.contributor.author Van Heerden, Esta
dc.contributor.author Piater, Lizelle A.
dc.contributor.author Litthauer, Derek
dc.date.accessioned 2012-10-24T07:22:39Z
dc.date.available 2012-10-24T07:22:39Z
dc.date.issued 2011
dc.identifier.citation Abbai, Nathlee S. (et.al.) 2011. A deep gold mine metagenome as a source of novel esterases. African Journal of Biotechnology, 10(32): 6090-6100 en_US
dc.identifier.issn 1684–5315
dc.identifier.uri http://hdl.handle.net/10210/7898
dc.description.abstract New sources of enzymes for biotechnological applications are continually being sought for. While diverse microbial ecosysyems have been demonstrated in the deep subsurfaces, deep mines provide easy access to these specialist communities. Therefore, the aim of this study was to assess a deep mine biofilm as a source of novel esterase enzymes. Biofilm was collected from the Beatrix Mine in South Africa, at a depth of 808 m. Assessment of the diversity revealed a group of previously uncultured bacteria and archaea. A metagenome library was screened for esterolytic activity, producing two esterolytic clones: a phospholipase patatin protein and an isochorismatase family protein. The isochorismatase family protein contained the catalytic Asp and Cys but not the Arg, which is considered as important for catalysis. The patatin showed 55% similarity to its closest relative; the patatin family protein from Plesiocystis pacifica. The expressed patatin displayed a preference for the C6 ester and was maximally active at pH 8 and 30°C. This study reported that screening of a relatively small metagenome library from the deep mine biofilm provided two esterolytic clones, which differed from their known counterparts. This indicates that the deep mine ecosystems contain an untapped resource of novel and potentially useful enzymes which may have applications in chemical syntheses. en_US
dc.language.iso en en_US
dc.publisher Academic Journals en_US
dc.rights © Academic Journals, 2011. The final publication is available at: http://www.academicjournals.org/AJB, DOI: 10.5897/AJB10.1211 en_US
dc.subject Lipolytic activity en_US
dc.subject Biofilms en_US
dc.subject Esterases en_US
dc.title A deep gold mine metagenome as a source of novel esterases. en_US
dc.type Article en_US

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